Fig 4.

Subcutaneous inoculation of C57BL/6 mice with wild-type (WT) Y. pestis CO92, the ΔyapJ mutant, the complemented yapJ mutant, the ΔyapK mutant, or the complemented yapK mutant. Mice were injected with ∼10² CFU of the above-described strains in the scruff of the neck and were sacrificed at 60, 72, and 96 hpi to determine the colonization of cervical lymph nodes (A), spleen (B), and lungs (C). Each symbol represents a single mouse. Lymph node data are the composites of data from two independent experiments, and spleen and lung data are the composites of data from five independent experiments. Black bars indicate the median CFU recovered per gram of tissue for each group, dashed lines indicate the limit of detection for each tissue, and the X symbols indicate dead mice. No significant differences between groups were present when subjected to a Mann-Whitney t test with a two-tailed nonparametric analysis.