Table 2.

Primers used in this work

Primer	Description	Sequencea (5′–3′)
JDL65	yapK 5′ forward	ACGCGTCGACTGTTAGAAACTTCCATTACCCCCCCC
JDL66	yapK 5′ reverse	CGGATCCCATAAATGTACTCTATTAATTGCCTGAATTTATCGC
JDL67	yapK 3′ forward	GAAGATCTTAAATCGTTTTATATTAGCGGGTAAATCGTTTTATAGC
JDL68	yapK 3′ reverse	ATAAGAATGCGGCCGCATACGGAGTACCGCTATTTTGTTGC
JDL69	yapJ 5′ forward	ATAAGAATGCGGCCGCGTCTCCGCAAGTCTGGGGATTAGTCCGGT
JDL70	yapJ 5′ reverse	CGGATCCCATAGAATTACCCTATTAGCTGCCCC
JDL71	yapJ 3′ forward	CGGATCCTAATACAGGACGGTTTTGTTATATCGGG
JDL72	yapJ 3′ reverse	ACGCGTCGACGCGTTTATTAAGTAAAAAATTCTCTATGAGATAACCCTA
JDL168	YPTB3286 5′ forward	ACGCGTCGACGATAGATCGTGAACCCGATTTGAAC
JDL169	YPTB3286 5′ reverse	CGGATCCCATAAATGTACTCTATTAATTGCCTGAATTTATCG
JDL170	YPTB3286 3′ forward	CGGATCCTAAGTCGTTTTTATGTGTAAAGGGTATCAG
JDL171	YPTB3286 3′ reverse	ATAAGAATGCGGCCGCGGTATAAGGCAGAGTACAGAAGGA
JDL172	YPTB3285 5′ forward	ACGCGTCGACCAGGGGAGAATTTCAACATCCTG
JDL173	YPTB3285 5′ reverse	CGGATCCCATAGGGTACCTTATTCATTGTAACTGTC
JDL174	YPTB3285 3′ forward	CGGATCCTAAATCGTTTTTATGTGTAAAGGGTATCAGT
JDL175	YPTB3285 3′ reverse	ATAAGAATGCGGCCGCCTGTCAGCAAACATATCTGCTCC

^aRestriction sites are underlined.