Fig 5.
Innate type 2 responsiveness in the intestinal tract is not compromised by CD11c+ cell depletion. (A and B) RT-PCR for Ym-1 (Chi3L3) (A) and RELM-β (B) mRNA expression in intestinal homogenates from PBS- and DTx-treated uninfected and infected (Nb) mice. (C) Eosinophilia (SSChigh cells) in the MLNs of PBS- and DTx-treated uninfected and infected (Nb) mice. (D and E) Intracellular IL-4 and IL-13 expression by CD3− CD19− NBNT cells in MLNs of PBS- and DTx-treated uninfected and infected (Nb) mice. (F) Surface phenotype of CD3− CD19− IL-13+ NBNT cells in N. brasiliensis-infected mice given PBS (filled black) or DTx (filled dark gray) compared to a positive control (black line) or an isotype control (filled light gray). Data presented are means from 4 or 5 individual mice/group and are representative of two similar experiments; tissues from infected mice were harvested at day 7 postinfection. Statistically significant differences are shown as * (P < 0.05), ** (P < 0.01), or *** (P < 0.001).