Fig 4.

Quantification of mRNA for ROP1, GRA2, GRA7, and MAG1 in the brains of T. gondii-infected CBA/J mice treated or not with sulfadiazine. Two groups of CBA/J mice were infected with 10 cysts of the ME49 strain of T. gondii, and one group (n = 8) was treated with sulfadiazine beginning at 3 weeks after infection for the entire period of the experiment to prevent proliferation of tachyzoites during the chronic stage of infection. Another group of mice (n = 12) did not receive the treatment. Brain samples were obtained from both groups of animals at 2 months after infection. cDNA was prepared from the brains of these animals, and amounts of mRNA for ROP1, GRA2, GRA7, and MAG1 were measured by real-time reverse transcription-PCR (see Materials and Methods). Results are shown as the mean ± SE of each group. **, P < 0.001.