Fig 3.

Intracellular cell signaling pathway inhibition. TLR2-HEK cells were pretreated for 1 h with 25 μM p38 inhibitor SB03580, the ERK1/2 inhibitor U0126, the JNK inhibitor SP600125, and 25 μg/ml of the NF-κB inhibitory ligand prior to stimulation with 10 μg/ml of the indicated PorB molecules, 100 ng/ml of Pam₃CSK₄ or 20 ng/ml of TNF-α. IL-8 was measured in the cell supernatant by ELISA and is expressed as a percentage (mean ± standard error) of that induced by each stimulation in the absence of inhibitors. *, P < 0.05, and **, P < 0.005, by Wilcoxon signed rank test; n = 6.