Fig 5.

BEAS-2B cell activation. BEAS-2B cells were incubated with wtPorB, PorB loop mutants, or N. lactamica PorB (all at 10 μg/ml), Pam₃CSK₄ (100 ng/ml), TNF-α (20 ng/ml), and LPS (100 ng/ml) in FBS-free medium for 18 h. (A) Secretion of IL-8 measured by ELISA and expressed in pg/ml (mean ± standard error). P values relative to wtPorB: ****, P < 0.0001 by unpaired t test; n > 15. P values relative to N. lactamica PorB: **, P = 0.002. P values relative to Pam₃CSK₄: ***, P = 0.0002; ****, P < 0.0001. (B) Secretion of RANTES measured as described for panel A and expressed as fold change (in pg/ml) relative to nonstimulated cells (mean ± standard error). P values relative to Nlac PorB: ***, P < 0.0005, and **, P < 0.009, by unpaired t test; n = 6.