BEAS-2B cell activation by whole bacteria. BEAS-2B cells were incubated with whole Neisseria organisms (MOI of 10 CFU/cell) for 18 h in antibiotic-free medium, and IL-8 secretion was measured by ELISA of culture supernatants. Strains used include the N. meningitidis H44/76 parent strain, N. meningitidis H44/76 Δ1Δ4, N. meningitidis H44/76 Δ1Δ4 expressing N. lactamica PorB (Nme H44/76 Δ1Δ4-[Nlac PorB]), and N. lactamica Y92-1009. IL-8 is expressed in pg/ml (mean ± standard error). ***, P < 0.0001, and **, P < 0.0058, by unpaired t test; n = 9.