Table 1.
E. coli strains, primers, and plasmids used in this study
| Strain, plasmid, or primer | Relevant information or sequence | Reference(s) |
|---|---|---|
| E. coli strains | ||
| RS218 | E. coli K1 RS218 isolated from the cerebrospinal fluid of a neonate with meningitis | 43, 49 |
| L346 | RS218 with a lacZ deletion | This study |
| L365 | RS218 with a prc lacZ double deletion | This study |
| Δprc-RS218 | RS218 with a prc deletion | This study |
| Plasmids | ||
| pCL1920 | Low-copy-no. plasmid | 26, 42 |
| pCL1920-prc | pCL1920 harboring the prc gene, which is under the control of the promoter on the plasmid | This study |
| pTR147 | pTrc99A harboring a DNA fragment encoding C-terminally His6-tagged Prc | 39 |
| pTR163 | pTrc99A harboring a DNA fragment encoding the C-terminally His6-tagged Prc K455A variant | 39 |
| Primers | ||
| NK-prc-F | 5′-AGGCTTACCGCGTTAGCTGGCCTGCTTGCAATAGCAGGCCAGACCTTCGCCATATGAATATCCTCCTTAG-3′ | This study |
| NK-prc-R | 5′-CAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGAGTGTAGGCTGGAGCTGCTTC-3′ | This study |
| NK-lacZ-F | 5′-GTATTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCATATCAATATCCTCCTTAG-3′ | This study |
| NK-lacZ-R | 5′-GACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGTGTAGGCTGGAGCTGCTTC-3′ | This study |