Fig 5.
Immunization of mice with the CoaNM/vWbpNM vaccine and spectrum of disease protection against different S. aureus isolates. (A) Recombinant CoaNM/vWbpNM or mock (PBS) vaccine was used to immunize BALB/c mice (n = 5) with a prime-boost regimen. Immune sera were analyzed by an ELISA for the reactivity of mouse serum IgG toward purified CoaNM and vWbpNM. (B to F) Cohorts of BALB/c mice (n = 10) were immunized with a prime-boost regimen of purified CoaNM/vWbpNM or mock vaccine and challenged by intravenous injection with S. aureus USA300 (B), N315 (C), MW2 (D), CowanI (E), or WIS (F). The survival of animals was monitored over 10 days.