Fig 7.

Immunization of mice with the Coa₄/vWbp₂ vaccine and spectrum of disease protection against different S. aureus isolates. (A) Coa₄/vWbp₂ or mock (PBS) vaccine was used to immunize BALB/c mice (n = 5) with a prime-boost regimen. Immune sera were analyzed by an ELISA for the reactivity of mouse serum IgG toward purified Coa₄ and vWbp₂. (B to F) Cohorts of BALB/c mice (n = 20 in panel B and n = 10 in panels C to F) were immunized with a prime-boost regimen of purified Coa₄/vWbp₂ or mock vaccine (B) and challenged by intravenous injection with S. aureus USA300 (B), N315 (C), MW2 (D), CowanI (E), or WIS (F). The survival of animals was monitored over 10 days.