Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | Cloning strain; hsdR recA endA | 36 |
| BW25141 | Strain for maintenance of conditional replicative oriRγ plasmids | 20 |
| S17λpir | Strain used for conjugation | 62a |
| Y. pestis | ||
| KIM6+ | Attenuated biovar 2.MED strain; pgm+, pCD1− | 68 |
| KIM6+ Δy0350 | Deletion of usher gene y0350 in KIM6+ | This study |
| KIM6+ Δy0562 | Deletion of usher gene y0562 in KIM6+ | This study |
| KIM6+ Δy1858 | Deletion of usher gene y1858 in KIM6+ | This study |
| KIM6+ Δy1871 | Deletion of usher gene y1871 in KIM6+ | This study |
| KIM6+ Δy2390 | Deletion of usher gene y2390 in KIM6+ | This study |
| KIM6+ Δy3480 | Deletion of usher gene y3480 in KIM6+ | This study |
| KIM5+Ap | Fully virulent biovar 2.MED strain; KIM6+/pCD1Ap | 34 |
| KIM5+Ap Δy0350 | Deletion of usher gene y0350 in KIM5+ | This study |
| KIM5+Ap Δy0562 | Deletion of usher gene y0562 in KIM5+ | This study |
| KIM5+Ap Δy1858 | Deletion of usher gene y1858 in KIM5+ | This study |
| KIM5+Ap Δy1871 | Deletion of usher gene y1871 in KIM5+ | This study |
| KIM5+Ap Δy2390 | Deletion of usher gene y2390 in KIM5+ | This study |
| KIM5+Ap Δy3480 | Deletion of usher gene y3480 in KIM5+ | This study |
| Plasmids | ||
| pGEM-T Easy | Cloning vector; Ampr | Promega |
| pMMB91 | Expression vector, IPTG-inducible promoter; Kanr | 23 |
| py0350 | Usher gene y0350 in pMMB91 | This study |
| py1858 | Usher gene y1858 in pMMB91 | This study |
| py1871 | Usher gene y1871 in pMMB91 | This study |
| pKD4 | FRT sites; Kanr Ampr | 20 |
| pKOBEG-sacB | λ phage redγβα, arabinose inducible; Clmr | 12, 50a |
| pFLP2 | sacB FLP-λpR; Ampr | 39 |
| pCD1Ap | pCD1 virulence plasmid with bla; Ampr cassette | 34 |
| pGFP | gfp3.1 in vector pMMB207, IPTG inducible; Ampr Clmr | 35 |
a
Clmr, chloramphenicol resistance; Ampr, ampicillin resistance; Kanr, kanamycin resistance.