Fig 2.
(A) Coomassie-stained SDS-PAGE gel (12%) of rSes. Lane 1, SDS-PAGE marker; lane 2, total E. coli extract after induction of SesC expression and cell lysis; lane 3, protein extract after binding the His-tagged SesC to the nickel column; lane 4, protein extract after washing the nickel column; lane 5, SesC recombinant protein after elution; lane 6, negative control. (B) Affinity of preimmune (□) and αSesC-IgGs (■) to rSesC. An indirect ELISA was performed using a 96-well ELISA plate coated with rSesC. IgGs were added to each well and incubated for 3 h at 37°C. Bound IgGs were measured at OD405 with an alkaline phosphatase-conjugated anti-rabbit immunoglobulin. x and y axes indicate the log10 IgG concentration (ng/ml) and the OD405 absorbance, respectively.