Fig 3.

Effect of total IgGs purified from preimmune (□) and anti-Ses (L, K, M, B, and C) and anti-whole-cell (WC) sera (■) on primary attachment (A) and overnight biofilm formation (B) of S. epidermidis strain 10b on a polystyrene surface. Overnight cultures were diluted, mixed with IgGs, incubated for 2 h at 4°C, and then transferred into the wells. After 2 h (A) or 14 h (B) of incubation at 37°C, the plates were washed and stained with crystal violet, and the OD₅₉₅ was measured. The error bars indicate the standard deviations. The data are the averages of eight measurements in two independent experiments. Biofilm inhibition was as defined in the text.