Table 1.

Primers used in this study

Primer	Sequence (5′–3′)a	Useb
sesCF1	GTTGATAACCGTCAACAAGG	A
sesCR1	CATGTTGATCTTTTGAATCCC	A
sesLF1	TGGGCCACTCAATACAGTCA	A
sesLR1	TTGGCGTGTTTCTGTCTTTG	A
sesMF1	CAGGTGCCTTGGAATCGC	A
sesMR1	GCGTACCTTGCCAGTAGTC	A
sesKF1	CCAATTACTAGTATTAAATTCAG	A
sesKR1	CTACACTGTTAGACGTGAG	A
sesBF1	GCTATGAAAAATAGTGGTGGC	A
sesBR1	CGTAGTATGAATTGAGCTCAC	A
sesCF2	ACGTGCTAGCGCAGATTCAGAAAGTACATC	B
sesCR2	GAACAGCTACAGCTGATCATCACCATCACCATCACTAGGATCCGCAT	B
sesLF2	CACGTGCTAGCCATCACCATCACCATCACAAAACGCAAGATGAAGCGAAA	B
sesLR2	GGAACTCAAATTATTTATTAAGGATCCGCAT	B
sesMF2	ACTGGCTAGCCATCACCATCACCATCACGGGGGCACCTCAAGTACAG	B
sesMR2	GTTACACCAGAATCTATCTATTAGGATCCGCAT	B
sesKF2	CACGTGCTAGCGCTGAATCAAACACTTCAGTTTCTTCT	B
sesKR2	CTATTACCAAATACAGGTATGCATCACCATCACCATCACTAGGATCCGCAT	B
sesBF2	ACGTGCTAGCGCAGCCGAAGTAACATCTC	B
sesBR2	CTCAATTCATACTACGTAGGTCATCACCATCACCATCACTAGGATCCGCAT	B

^aFlanking restriction sites are underlined and in italics, and sequences coding for an N- or C-terminal His₆ tag are indicated in boldface.

^bA, used for PCR screening of ses genes in clinical and commensal isolates; B, used for cloning ses genes in pET11c.