Fig 1.

(A) Viability of wild-type, Tx3, and Tx5 N. brasiliensis by plate counting. (B) Inflammation area (in cm³) in the footpad of mice infected with wild-type, Tx3, or Tx5 N. brasiliensis. (C) Kinyoun staining of wild-type and Tx5 N. brasiliensis. N. brasiliensis loses its acid-fastness after benzine treatment. (D) Transmission electron microscopy images of wild-type and Tx5 N. brasiliensis. A disruption in the integrity of the cell envelope is observed after benzine treatment. The outmost electrodense layer is missing from the Tx5 N. brasiliensis cell wall.