Fig 3.

Few 6N+ macrophages in Salmonella-infected mice contain broken DNA, a marker of apoptotic or dead cells. (A) Dot plots illustrating gating for BrdU⁺ cells from control, Salmonella-infected (1 week postinfection), and Yersinia-infected mice (1 week postinfection). (B) The percentage of 2-4N (squares) and 6N+ (circles) CD68⁺ cells containing broken DNA (BrdU⁺) in control (white), Salmonella-infected (black), and Yersinia-infected (gray; at 1 week) mice. Means and SEM from two experiments are shown. n = 3 to 4 at each time point for control mice, n = 12 at each time point for Salmonella-infected mice, and n = 9 for Yersinia-infected mice. P values compare each 6N+ data point to the corresponding control mice. *, P < 0.005; +, P < 0.05; each by one-way ANOVA with Tukey's posttest. More than 75% of CD68⁺ cells are macrophages (CD68⁺ CD11c^low/− Gr-1^int/−).