Fig 9.

Identification of mitochondrion-associated genes that impact E. chaffeensis infection. san [N-(alpha) acetyltransferase 50] and Uck2 (uridine/cytidine kinase) are needed for the synthesis of cardiolipin. Phosphatidic acid (PA), a product of san activity, and CTP, which is produced from CMP, a product of Uck2, are both necessary substrates for biosynthesis of cardiolipin, the major inner mitochondrial membrane lipid. Cht11 (chitinase 11) encodes part of a protein transporter in the inner mitochondrial membrane. whd (withered) encodes carnitine palmitoyltransferase 1, and Echs1 encodes enoyl-CoA hydratase; both enzymes are needed for the production of acetyl-CoA. Ccdc58 encodes coiled-coil domain-containing protein 58, which is found in complex with the inner mitochondrial membrane molecules MRPS28 and SSBP1. Apopt1 (Apoptogenic 1) induces the release of cytochrome c through the permeability transition pore and triggers apoptosis. Nup37 (Nucleoporin 37) is not found in the mitochondrion but is part of a nucleopore complex that transports hexose molecules, including glucose, an important molecule in energy metabolism.