Fig 2.

The five L. pneumophila effectors cause upregulation of MAPK-dependent host genes. (A to C) TKO macrophages were infected for 4 h with the indicated strains of L. pneumophila. A p38 inhibitor (SB203580 [SB]; 1 μM) was added 30 min before infection, where specified. Levels of Il1a (A), Dusp1 (B), and Fos (C) mRNA were measured by quantitative RT-PCR. (D) B6 macrophages were infected with L. pneumophila ΔflaA for 24 h alone or in the presence of p38 inhibitor (1 μM), JNK II inhibitor (1 μM), or both (1 μM each). IL-1α protein in the supernatant was measured by ELISA. Data shown are representative of three experiments (mean ± SD for panels A to C).