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EIC’s for the deuterated (triangle) and nondeuterated (square) peaks of LNT with the H/D ratio (circle) calculated from each point along the peak. A. The H/D ratio increases across the peak because the deuterated peak elutes one second before the nondeuterated peak. B. The H/D ratio is constant across the peak after shifting the deuterated peak intensities to artificially co-elute with the nondeuterated peak.
