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. 2012 Aug 29;32(35):12152–12164. doi: 10.1523/JNEUROSCI.0628-12.2012

Figure 3.

Figure 3.

PEDF infusion increases the numbers of SVZ cells expressing early oligodendroglial lineage markers or oligodendroglial transcription factors. Saline or PEDF (300 ng per day) was administered via osmotic pump into the lateral ventricle of adult GFAP:GFP transgenic mice for 7 d. The SVZ tissues were double-immunostained for GFP and markers for early oligodendroglial lineage (PDGFrα or NG2) or oligodendroglial transcription factors (Olig1, Olig2, or Sox10). A–F, Confocal images of coronal sections of saline- and PEDF-infused SVZ immunolabeled with antibodies against GFP and PDGFrα, NG2, or Olig1. Insets show magnified images of areas indicated by rectangles. Scale bar, 30 μm. G–K, Percentages of DAPI+ nuclei labeled with PDGFrα+, PDGFrα+GFP+, and PDGFrα+GFP− (G), NG2+, NG2+GFP+, and NG2+GFP− (H), Olig1+, Olig1+GFP+, and Olig1+GFP− (I), Olig2+, Olig2+GFP+, and Olig2+GFP− (J), and Sox10+, Sox10+GFP+, and Sox10+GFP− (K) in the saline- and PEDF-infused SVZ. PEDF administration increased the proportions of both GFP+ and GFP− cells coexpressing these oligodendroglial markers or transcription factors. Results are means ± SEM (n = 4–5 brains). *p < 0.05, **p < 0.01, and ***p < 0.005, Student's t test.