Figure 7.

PEDF enhances oligodendrocyte production and maturation of cycling OPCs in the adult corpus callosum. A, Experimental design. Adult wild-type mice received three consecutive administrations of BrdU (100 mg/kg body weight, i.p.) at 6 h intervals to label cycling OPCs in the corpus callosum, followed by saline or PEDF (300 ng per day) intracerebral infusion for 9 d. B, C, Confocal images of cells double-immunostained for BrdU and Olig2 (B) or CC1 (C) at day 0. D–F, Confocal images of cells double-immunostained for BrdU and CC1 (D) or CNP (F) in PEDF-infused corpus callosum, and for BrdU and CNP (E) in saline-infused corpus callosum at day 9. G, Percentages of BrdU+ cells that were colabeled with Olig2 at day 0 and day 9. The vast majority of cycling cells in the corpus callosum were Olig2+. H, Quantification for cells coimmunolabeled for BrdU and CC1, a marker for mature oligodendrocytes, in the corpus callosum at day 0 and day 9. At day 0, BrdU-labeled OPCs did not express CC1. By day 9, CC1+ mature oligodendrocytes had been produced from BrdU+ OPCs after both saline and PEDF infusion, but significantly more with PEDF infusion. I, Percentages of BrdU+ cells colabeled with CNP in saline- and PEDF-infused corpus callosum demonstrating that PEDF robustly promoted terminal maturation of newly born OPCs. ND, Not detected. B–F, Arrows indicate BrdU+ cells coimmunostained with oligodendroglial markers. Scale bar, 35 μm. Results are means ± SEM (n = 3 brains). *p < 0.01, an increase in the number of BrdU+CC1+ cells in between saline- vs PEDF-infused corpus callosum at day 9, Student's t test.