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. 2012 Aug 29;32(35):12152–12164. doi: 10.1523/JNEUROSCI.0628-12.2012

Figure 9.

Figure 9.

PEDF promotes oligodendroglial regeneration in lysolecithin-induced demyelinative corpus callosum. Administration of lysolecithin to adult corpus callosum to induce focal demyelination was followed by saline or PEDF (300 ng per day) infusion for 3 or 7 d. A–H, Confocal images of cells in saline- or PEDF-infused corpus callosum immunolabeled with antibodies against NG2 (A–D), CC1 (E, F), or CNP (G, H) at 3 or 7 dpl. Scale bars, 25 μm. I–L, MBP immunostaining of saline (I, K)- or PEDF (J, L)-infused corpus callosum at 7 dpl. K, L, High-magnification images of the areas indicated by rectangles in I and J, respectively. Scale bars, 50 μm. M, N, Quantification for NG2+ OPCs at 3 and 7 dpl (M), and CC1+ and CNP+ mature oligodendrocytes (N) at 7 dpl. Note that PEDF infusion resulted in markedly higher numbers of NG2+ cells present in the lesional corpus callosum at 3 dpl, and substantially enhanced CNP+ mature oligodendrocyte production and CNP immunoreactivity, and MBP-positive myelin levels at 7 dpl. CC, Corpus callosum; LV, lumen of lateral ventricles. Results are means ± SEM (n = 4 brains). *p < 0.05 and **p < 0.01, Student's t test.