Table 2.

20S Proteasome Chymotrypsin-like Activity

	% Vehicle a
	0 Hr	2 Hr	16 Hr	24 Hr
TRK43-VECTOR
Vehicle	100	100	100	100
10 μM HNE	96 ± 8	57 ± 6 †	54 ± 10 †	137 ± 9 †
20 μM HNE	65 ± 5 †	49 ± 7 †	53 ± 7 †	225 ± 7 †
TRK43-ALDH3A1
Vehicle	100	100	100	100
10 μM HNE	98 ± 3	123 ± 8 †	157 ± 5 †	106 ± 10
20 μM HNE	91 ± 5	139 ± 7 †	164 ± 4 †	116 ± 14

^a20S proteasome chymotrypsin activity was measured in lysates of TRK43 cells stably transfected with human ALDH3A1 or empty vector following 1 hr treatment with 4-HNE (10 or 20 μM) or vehicle and 0, 2, 16 or 24 hrs recovery. Data are expressed as a percentage of the levels in vehicle treated (vehicle) cells. Values are expressed as mean ± SD from triplicate experiments.

^†P < 0.05 one-way ANOVA compared with the Control cells in the same transfectant population at the same time point.