Figure 5.
(A and B) Manual pipette-based, pump-free separation of HIV particles from whole blood using the filter microchip. (A) Whole blood samples spiked with HIV at concentrations of 103, 104, and 105 copies/mL, and (B) discarded HIV patient whole blood samples were flowed through filter microchips with membrane pore size of 1 μm or 2 μm.
Notes: Subsequently, blood samples containing HIV particles were manually washed with 300 μL of phosphate-buffered saline and all the filtrate (approximately 200 μL) was collected. HIV recoveries (%) in both 1 μm and 2 μm filter microchips were then calculated using formula 2. Data are presented as average ± standard error (n = 6). One-way analysis of variance was performed. *P < 0.05; in B, x-axis is log-scaled.