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. 2012 Sep 17;2012:508972. doi: 10.1155/2012/508972

Figure 4.

Figure 4

Immunoblotting of LEG-1 from the treated fibroblasts and the measurement of ROS within the treated fibroblasts. The immunoblot using LEG-1 antibody showed that LEG-1 (spot 17) was only significantly downregulated in cells that had undergone the LR20 treatment (a). On the other hand, GSTP-1 (B, spot 1, Figure 3, cluster F) and PRDX-2 (C, spot 3, Figure 3, cluster F) were upregulated in cells that underwent either LR treatment, which was confirmed by immunoblotting with specific antibodies. However, only overexpression of PRDX-2, but not overexpression of GSTP-1, was detected in cells after S100 treatment (c). The ROS assay showed that ROS production was significantly reduced in cells treated with LR20 (d). GAPDH was employed as the sample loading control. The related expression levels were detected by Fujifilm Multigauge ver. 2.0 and a Student's  t-test was used to evaluate the statistical significance. Results are from triplicate experiments and are presented as the mean ± SD (n = 3). *P < 0.05; **P < 0.01.