Figure 5.

The Boyden chamber migration test and the wound healing assay were used to detect the motility of fibroblasts after the various drug treatments. The migration assay showed that LR5-, LR20-, and S100-treated cells underwent less migrated through the chamber (a). The wound healing assay further demonstrated that treatment with the LR extracts was able to significantly decrease the migration of cells in a dose-dependent manner (b). Furthermore, the active component S100 was also able to create a similar effect on cell migration. Quantification of the chamber migration (c) indicated that LR20 significantly reduced cell migration and to the largest extent. Quantification of the wound area (d) showed that fewer cells migrated into the wound area when the cells were treated with LR20. The results are expressed as mean ± SD (n = 3). *P < 0.01; ***P < 0.001, compared with DMSO.