Table 1.
Proteins showing altered abundance after treatment with either the LR extracts or shikonin compared to the DMSO control and identified by LC-MS/MS.
| ID | Protein identity | Accession number |
Abbreviation | MW/pI | Score | Match peptide |
Sequence coverage (%) |
LR 5/DMSO | LR 20/DMSO | S100/DMSO |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Glutathione S-transferase P | P09211 | GSTP1 | 23/5.43 | 224 | 8 | 46 | ↑ 2.26 | ↑ 2.66 | ↑ 1.61 |
| 2 | Eukaryotic translation initiation factor 5A-1 | P47942 | IF5A1 | 17/5.08 | 58 | 4 | 23 | ↑ 1.17 | ↑ 1.30 | ↓ 0.96 |
| 3 | Peroxiredoxin-2 | P32119 | PRDX2 | 22/5.38 | 143 | 6 | 23 | ↑ 1.65 | ↑ 1.84 | ↓ 0.89 |
| 4 | Actin, cytoplasmic-1 | P60711 | ACTB | 42/5.29 | 136 | 4 | 8 | ↓ 0.44 | ↓ 0.89 | ↓ 0.22 |
| 5 | T-complex protein 1 subunit beta | P78371 | TCPB | 36/8.43 | 169 | 12 | 21 | ↓ 0.88 | ↓ 0.68 | ↓ 0.66 |
| 6 | Glutaredoxin-3 | O76003 | GLRX3 | 37/5.31 | 81 | 8 | 19 | ↓ 0.61 | ↓ 0.69 | ↓ 0.83 |
| 7 | Translationally controlled tumor protein | P13693 | TCTP | 20/4.84 | 119 | 4 | 17 | ↓ 0.66 | ↓ 0.40 | ↓ 0.39 |
| 8 | Cofilin-1 | P23528 | COF1 | 18/8.22 | 113 | 7 | 24 | ↓ 1/∞ | ↑ 9.67 | ↓ 1/∞ |
| 9 | Serum albumin | P02768 | ALBU | 69/5.92 | 119 | 2 | 3 | ↑ 1.24 | ↑ 1.78 | ↑ 1.04 |
| 10 | Microtubule-associated protein RP/EB family member 1 | Q15691 | MARE1 | 30/5.02 | 65 | 3 | 17 | ↓ 0.85 | ↓ 0.61 | ↓ 0.61 |
| 11 | Chloride intracellular channel protein 1 | O00299 | CLIC1 | 27/5.09 | 114 | 3 | 12 | ↓ 0.89 | ↓ 0.64 | ↓ 0.69 |
| 12 | Superoxide dismutase | P00441 | SODC | 16/5.70 | 61 | 1 | 9 | ↑ 1.02 | ↑ 1.54 | ↓ 0.91 |
| 13 | Nucleoside diphosphate kinase A | P15531 | NME1 | 17/5.83 | 63 | 2 | 5 | ↑ 1.20 | ↑ 1.30 | ↓ 0.92 |
| 14 | Actin-related protein 2/3 complex subunit 5 | O15511 | ARPC5 | 16310/5.47 | 179 | 6 | 27 | ↓ 0.51 | ↓ 0.97 | ↓ 0.49 |
| 15 | Stathmin | P16949 | STMN1 | 17292/5.76 | 134 | 6 | 36 | ↑ 2.54 | ↑ 1.98 | ↑ 1.99 |
| 16 | 1,2-Dihydroxy-3-keto-5-methylthiopentene dioxygenase | Q9bv57 | ADI1 | 21484.6/ | 2.5 (criteria) | 4 | 22 | ↑ 1.75 | ↑ 2.21 | ↑ 1.22 |
| 17 | Galectin-1 | P09382 | LEG1 | 14706/5.34 | 81 | 3 | 19 | ↓ 0.39 | ↓ 0.55 | ↓ 0.28 |
| 18 | Proteosome activator complex subunit 1 | Q06323 | PSME1 | 28705/5.78 | 289 | 7 | 27 | ↑ 3.12 | ↑ 2.64 | ↑ 2.37 |
| 19 | Annexin A5 | P08758 | ANXA5 | 35914/4.94 | 2.5 (criteria) | 16 | 65 | ↑ 2.31 | ↑ 1.68 | ↑ 1.98 |
| 20 | Peroxiredoxin-4 | Q13162 | PRDX4 | 30521/5.86 | 105 | 4 | 14 | ↑ 2.81 | ↑ 2.27 | ↑ 2.51 |
| 21 | Phosphoglycerate kinase 1 | P00558 | PGK1 | 44586/8.30 | 94 | 1 | 4 | ↓ 0.45 | ↓ 0.43 | ↓ 0.41 |
| 22 | 14-3-3 protein epsilon isoform | P62258 | YWHAE | 26504/4.76 | n/a | n/a | ↑ 1.86 | ↑ 1.31 | ↑ 1.58 |