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. 2012 Oct;140(4):391–402. doi: 10.1085/jgp.201210798

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© 2012 Dürrnagel et al.

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Figure 4. — Preventing a rise in [Ca²⁺]_i changes E_rev of sustained peptide-activated currents. I/V relations for HyNaC2/3/5 activated by 1 µM Hydra-RFamide I in standard bath (open circles), in standard bath after injection of EGTA (closed squares), and in standard bath in which CaCl₂ had been replaced by BaCl₂ (closed triangles). Currents were normalized to the current measured at −70 mV, which had an amplitude of −4.4 ± 1.1 µA (Ca²⁺), −1.4 ± 0.2 µA (Ca²⁺/EGTA), or −6.0 ± 0.8 µA (Ba²⁺) (n = 7 - 8), respectively. The inset shows the I/V relations close to the E_revs on an expanded scale.