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. 2012 Oct;140(4):391–402. doi: 10.1085/jgp.201210798

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© 2012 Dürrnagel et al.

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Figure 5. — Activation of HyNaC increases [Ca²⁺]_i in oocytes. (A) Representative trace (black) from photometric Ca²⁺ measurements illustrating the increase of the F340/380 ratio in a HyNaC2/3/5-expressing oocyte after the application of RFamide I (asterisk). Oocytes had been injected with 50 nl Fura-2AM (1 mM) 30–120 min before the recording. Thus, the increased F340/380 ratio reflects an increase in [Ca²⁺]_i. The red trace represents the current that paralleled the rise in [Ca²⁺]_i. HyNaC was activated by pipetting Hydra-RFamide I into the bath to yield a final concentration of ∼1 µM RFamide I. (B) Changes of the F340/F380 ratio caused by activation of HyNaC for individual measurements (n = 8).