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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Cancer Discov. 2012 Jul 9;2(9):826–839. doi: 10.1158/2159-8290.CD-12-0107

Figure 2. Cultured fibroblasts recapitulate multiple phenotypes associated with their respective tissues of origin.

Figure 2

(A) 3 LDAFs and 3 HDAFs were placed under proliferative (ctl) or adipocyte differentiation (+PJ2) conditions for 6 days and assessed for fat accumulation by Oil Red O staining. Representative bright field images (10×) of the fibroblasts under differentiation conditions. (B) Average and SEM of Oil Red O staining per cell. (C) 4 LDAFs and 4 HDAFs were placed under proliferative (ctl) or adipocyte differentiation conditions (+PJ2) for 2 weeks and assessed for adipocyte differentiation by measuring the expression of genes upregulated in adipogenesis. Average and SEM of CD36 and LEP expression by QPCR (n= 4 for each of 4 LDAFs and 4 HDAFs). (D) Representative fluorescent images (10×) highlighting differential ECM protein deposition in 6 LDAFs and 6 HDAFs grown for 5 days and assessed for accumulation of matrix proteins COL1A1, FN1, OPN and TNC. (E) Left panels: representative bright field images (10×) of 1 RMF and 2 CAFs under proliferative (ctl) or adipocyte differentiation (+PJ2) conditions for 7 days and assessed for adipocyte formation by Oil Red O staining. Right panel: average and SEM of Oil Red O staining per cell.