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. 2012 Jun 11;18(10):477–488. doi: 10.1093/molehr/gas025

Figure 3.

Figure 3

LIN28 protein and mRNA in marmoset and mouse testes. (A) LIN28 is present in the cytoplasm of mouse gonocytes and (B and C) immature spermatogonia, as well as in adult spermatogonia (D). The scale bar represents 50 µm. Quantitative RT–PCR with marmoset (E) and mouse (F) whole testis RNA. (E) Newborn, 8 weeks-old, and adult marmoset testes. There is a strong and significant decrease in the relative mRNA abundance during testicular development. (F) The expression profile of the mouse testis shows a strong increase from birth to PND9 and subsequent decrease to adulthood. The adult relative expression levels were still above the newborn levels. (G) Western blot analysis of LIN28 in newborn and adult marmoset monkey testis and control tissues. Omission of the primary antibody resulted in no signal (data not shown). (H) Western blot analysis of LIN28 in neonatal, post-natal day 9 and adult mouse testes.