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. 2012 Sep 24;209(10):1743–1752. doi: 10.1084/jem.20112398

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© 2012 Hara-Chikuma et al.

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Figure 1. — Normal cellularity and subpopulations of T cells in AQP3-null mice. (a) The messenger RNA expression levels of AQP3 in sorted CD4⁺ and CD8⁺ T cells as well as in kidney and brain tissues were assessed by real-time PCR (SE; n = 4). Data are expressed as the AQP3/GAPDH ratio. (b) Flow cytometric analysis of AQP3 expression in CD4⁺ (top) or CD8⁺ (bottom) T cells from WT and AQP3^−/− mice. (c) Cell population analysis in the spleen (left) and LN (right). The numbers of total cells, CD4⁺ and CD8⁺ cells from WT and AQP3^−/− mice (SE; n = 4), are shown. (d) Cell population analysis in the thymus. The numbers of total cells and indicated subsets (SE; n = 4) are shown. (e) [³H]Thymidine incorporation in CD4⁺ T cells from WT and AQP3^−/− mice (SE; n = 4–5). Each experiment was performed three times.