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. 2012 Sep 24;209(10):1841–1852. doi: 10.1084/jem.20120219

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© 2012 Liu et al.

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Figure 7. — Memory Tfh cells promote humoral recall responses. (A) 30 d after immunization with KLH in CFA s.c., flow cytometry analysis of CXCR5 and Bcl6-RFP expression in activated T cells from Bcl6-RFP reporter mice was performed. Data are representative of two independent experiments. (B) Experimental design for functional analysis of mTfh and non-mTfh cells. (C) CXCR5⁺Bcl6^hi or CXCR5⁻Bcl6⁻ OT-II CD4⁺ T cells (CD45.1⁺) were purified 21 d after immunization and adoptively transferred at equal number (1.5 × 10⁵) into naive congenic recipients (CD45.2⁺). 7 d later, the phenotype of donor cells in the dLNs and their population were determined by flow cytometry. (D) Flow cytometry analysis of GL7 and Fas on B220⁺ B cells from mTfh and non-mTfh transferred recipients; the nontransfer mice were set as controls. (E) The percentage of GL7⁺Fas⁺ B cells in dLNs was quantified. (F) OVA-specific antibody levels (total IgG, IgG1, and IgG2a) were determined by ELISA. (C–F) Cumulative data are representative of two independent experiments displayed as mean ± SD. n = 4 per group. **, P < 0.01; ***, P < 0.001.