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. 2012 Sep 24;209(10):1825–1840. doi: 10.1084/jem.20120774

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© 2012 Chappell et al.

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Figure 9. — Ag-specific B cells primed by DCIR2⁺ DCs are highly efficient APCs for CD4 T cell proliferation. (A) Schematic showing experimental design. (B) Histograms show dilution of CFSE among gated CD4⁺Va2⁺ OT-II T cells after 72 h in culture with NP-specific B cells from spleens of B1-8^hi recipient C57BL/6 mice immunized 12 or 36 h previously with NP-33D1 or NP-rat2b. (C) Proliferation indices generated from the data in B are shown. (D) Same as in B, except sorted DCIR2⁺ DCs from spleens of immunized C57BL/6 mice were used as APCs. (E) Proliferation indices generated from the data in D are shown. Proliferation index is defined as total CFSE fluorescence of the negative control (APC/T cell ratio of 0:1) divided by total CFSE fluorescence of indicated samples. A representative experiment of two independent experiments for each time point is shown for both B and D. (F) Day 12 anti-NP IgG Ab responses in MHCII-deficient and C57BL/6 recipients of OT-II CD4 T cells and B1-8^hi B cells after immunization with NP-OVA-33D1 or NP-OVA-rat2b is shown. Each dot represents an individual animal with the mean (horizontal bars) indicated. A representative experiment of two independent experiments using four to five mice/group is shown. P-value indicator *** refers to P < 0.001.