Table 3.
Dual L-chain spontaneous hybridomas from MRL/lpr-Igkm/h mice
| Hybridoma | Isot. | React. | H chaina | L chaina | |||||
| VH | DH | JH | CDR3 | VL | JL | CDR3 | |||
| Dual-κ/λ | |||||||||
| LN 2A4b | IgM | Sm | VH7-3 (S107) | DH1-1 (DFL16.1) | JH2 | CARYVTTVPNFDYW (gl) | Vκ6-17 (Vκ19/28) | Jκ4 | CQQHYSTPFTF (gl) |
| Vλ1 | Jλ1 | CALWYSNHWVF (gl) | |||||||
| Dual-κ/κ | |||||||||
| LN 2A8c | IgG | Chr | VH1-7 (J558) | DH2-3 (DSP2.9) | JH3 | CARGWLLRTYW (12 mut) | Vκ12-46 (Vκ12/13) | Jκ1 | CQHFYGTPWTF (7 mut) |
| Vκ2-137 (Vκ24/25) | Jκ4 | CMQHLEYPFTF (gl) | |||||||
These two hybridomas were among 816 spontaneous B cell hybridomas generated from nonmanipulated spleen and LN cells of three individual MRL/lpr-Igkm/h mice (a total of six fusions). Isot., isotype; React., reactivity; Sm, Smith antigen; Chr, chromatin; gl, germline sequence; mut, number of predicted somatic mutations in the overall V(D)J sequence based on comparison with IMGT-deposited germline sequences.
Ig H and L chain gene segments are reported with the IMGT nomenclature. The old nomenclature is given in parenthesis under the IMGT nomenclature.
This hybridoma is representative of many clones (∼80% of the all hybridomas) isolated from both the spleen and LNs in all fusions.
This hybridoma was from the fusion of LN cells from one MRL/lpr-Igkm/h mouse.