Skip to main content
. Author manuscript; available in PMC: 2012 Sep 25.
Published in final edited form as: Oncogene. 2011 Mar 14;30(30):3381–3390. doi: 10.1038/onc.2011.50

Figure 4.

Figure 4

Prolactin production is not significantly altered in mice with the TRβPV mutation. (a) Prolactin serum levels in nulliparous mice of the six genotypes. The estrous cycle was monitored daily with a vaginal impedance checker (Muromachi Kikai, Tokyo, Japan)and females were bled when not at the proestrus stage to avoid the serum prolactin peak. Serum prolactin levels were measured by radioimmunoassays at the National Hormone & Peptide Program, Harbor-UCLA Medical Center (Dr AF Parlow). (b) Prolactin content in the pituitaries of nulliparous mice of the six genotypes. Pituitary lysates preparation and western blots were carried out following a procedure previously described (Guigon et al., 2008). The anti-prolactin antibody was purchased from the National Hormone & Peptide Program, Harbor-UCLA Medical Center. GAPDH (glyceraldehyde-3-phosphate dehydrogenase) was used as a loading control.