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. Author manuscript; available in PMC: 2012 Sep 25.
Published in final edited form as: Hepatology. 2010 Nov 9;52(6):2158–2166. doi: 10.1002/hep.23945

Figure 1. Tol2 transposon based enhancer trap screen.

Figure 1

(A) The enhancer trap constructs contain Tol2 transposon elements and a minimal promoter of the zebrafish gata2 gene fused to EGFP reporter gene. When they are inserted in the operating area of an enhancer element, the expression of the reporter gene can be induced and observed. (B) Tol2 insertion location of mp255c fish line. Tol2 elements were inserted at chromosome 18, 6.1 kb upstream of gene mmp23b. (C and E) mmp23b:GFP expression pattern. (C) Dorsal view at 3dpf. Note liver expression (white arrow). (E) Lateral view at 4dpf. (D and F) WISH using an mmp23b probe. (D) Dorsal view at 3dpf (100%, n=30). Note tissue specific expression in the developing liver (black arrow). (F) Lateral view at 4dpf (100%, n=30).