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. Author manuscript; available in PMC: 2012 Sep 25.
Published in final edited form as: Hepatology. 2010 Nov 9;52(6):2158–2166. doi: 10.1002/hep.23945

Figure 5. Mmp23b/MMP23B is involved in TNF shedding.

Figure 5

(A) Western blot of supernatants and cell lysates using an anti-FLAG antibody. Co-transfection with HTNF-3xFLAG and GFP serves as negative control. Co-transfection with HTNF-3xFLAG and either HMMP23B or MTACE lead to a significant increase of soluble TNF level in the supernatants. Normalization of the ration of secreted TNF vs. cell associated TNF is listed. Anti-tubuling was used as a loading control. (B) ELISA analysis of TNF levels in supernatants and cell lysates. Grey: TNF levels in supernatants. Black: TNF levels in cell lysates. Co-transfection of HMMP23B increased the supernatants TNF level to an extent comparable to that of MTACE co-transfection. (C) Ratio of secreted vs. cell associated TNF. Ratios were calculated from supernatants TNF divided by cell lysates TNF. Both HMMP23B and MTACE led to a significant increase of TNF release from cell membrane comparing to that of control cells (GFP and TNF co-transfection). Error bars represent standard deviation. *P<0.05.