Fig 3.

p53-dependent pathways were dysregulated in the rpl11 mutant. (A) Expression of total tp53 and Δ113Ntp53 was increased, ΔNtp63 expression was slightly (1·5-fold) increased, tp73 expression was not changed, qPCR, 48 hpf. In A-C, RNA was pooled from 30 mutant or sibling embryos (B) cdkn1a and ccng1 responsible for cell cycle suppression and pro-apoptotic puma and bax were upregulated in the rpl11 mutant. (C) After p53 was inhibited by a morpholino, expression of p53 targets returned to the wild-type level. (D) Expression of runx1 was rescued in the rpl11 mutant by p53 inhibition. (E,F) The rpl11 mutant embryos with suppressed p53 had more red blood cells at day 3·5. In A–C, representative of two and in D–F, three independent experiments are shown.