The rpl11 mutant upregulated some factors promoting proliferation. Early response genes such as fos, junb and stat3 showed higher expression when p53 was inhibited by a morpholino (junb
P < 0·05, fos and stat3
P < 0·01, Student’s t-test). Upregulation of genes involved in synthesis of non-coding RNA and ribosome biogenesis did not depend on p53. Example is brf1, involved in tRNA biosynthesis. qPCR, 48 hpf, RNA was pooled from 30 embryos. Representative of two independent experiments is shown.