Skip to main content
. Author manuscript; available in PMC: 2013 Jan 20.
Published in final edited form as: Circ Res. 2011 Dec 1;110(2):220–229. doi: 10.1161/CIRCRESAHA.111.251298

Figure 2. Evolutionarily conserved regions up1 and int2 are sufficient but not necessary for tg(−2.3etsrp:gfp) expression in endothelial cells.

Figure 2

(A and B) The conserved region up1 (A) or int2 (B) was placed in front of the minimal gata2 promoter driving GFP expression and germline transgenics generated. Both regions are sufficient to drive GFP expression in the developing vasculature as demonstrated by fluorescent images of whole embryo and trunk vasculature at 24hpf. (C) Up1 and int2 are not necessary for strong vascular expression from the etsrp promoter transgene. Deletion of both up1 and int2 from the GFP reporter lines does not eliminate vascular expression as demonstrated by tg(−1.8etsrpEx2:gfp), suggesting that more regulatory elements are present. The numbers in the whole embryo image represents the number of germline transgenic fish lines expressing vascular GFP versus the total number of lines examined. Note that up1 drives stronger expression in the dorsal aorta and its deletion results in decreased relative expression in the dorsal aorta (A and C).