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Setup and utilization of TopTips for SPE extractions. (A) Setup of a TopTip on a 2-ml centrifuge tube with an adaptor for centrifugation. (B) Process of liquid handling on a SPE TopTip. (C) Setup of a glass insert in a centrifuge tube for eluate collection. Corresponds to Step 45–46. (D) Process of methylating eluate in a glass insert. Corresponds to Steps 48–50.
