Table 1.
Summary of fatty acid and lipid biosynthetic genes in this study
| Gene | Category1 | Forward primer (5’–3’) | Reverse primer (5’–3’) | Accession no. |
|---|---|---|---|---|
| Ketoacyl ACP synthase I (KAS I) |
I |
TGGTTGGTCTTCTTTCCCTC |
ACAAACCCACCAGCTTCATAG |
JQ806261 |
| Ketoacyl ACP synthase II (KAS II) |
I |
ACAGTCACCTTGTTTTTGTTCC |
ATGCTAATTTACCCTGATAAGG |
JQ806262 |
| Ketoacyl ACP synthase III (KAS III) |
I |
CAATTATTAGATGGGGCTGAAG |
TATGTGACAACAGAAACCAAGC |
JQ806263 |
| 3-ketoacyl-CoA reductase isoform 1 (KCR1) |
I |
TCCTTTATTTGGGGTTTGTTAC |
AGCAACCTAAAAGTTCAATTCC |
JQ806264 |
| 3-ketoacyl-CoA reductase isoform 3 (KCR3) |
I |
ATGGACAGTAACATAGCCAATC |
TCCACACACTTTTTCACAACTG |
JQ806266 |
| Acyl carrier protein1 (ACP1) |
I |
ACCGTTCAGGAAGCTGCTG |
ACATAGTTCACAACAAGATTGC |
JQ806272 |
| Acyl-CoA dehydrogenase (ACD) |
I |
AATTGCAGATGGCCCCGATG |
AGTTTTTGTGTGCTGGTATTGG |
JQ806273 |
| Semialdehyde decarboxylase 1 (SAD1) |
I |
GGGGCAGTGATTTTCCATTTG |
GTGCTTTTTCCATATCTAATGAC |
JQ806274 |
| Hydroxyacyl-ACP dehydratase (HAD) |
I |
CTGCTATCCTATGCCTTTTTTG |
GTTTTGCCCATAAGTTTAACATC |
JQ806275 |
| Acyl-ACP thioesterase (FATA) |
I |
TATTTGTGTGGGCATCTGCC |
TAGTTGGTAAGGTGGGTTTAAC |
JQ806276 |
| Enoyl-ACP reductase (EAR) |
I |
ATGGGTGTGGGAGTTGACAG |
GTGACATGGCATGGATTAAATG |
JQ806277 |
| Phosphatidic acid phosphatase β (β-PAP) |
I |
CACGAGCCCCATTCTGGAC |
AGTCTGTTCAAGGTCAGGGG |
JQ806281 |
| CDP-diacylglycerol synthase (CDP-DAG) |
I |
CTAATAACAGTGTCATGGCAG |
TTCCATATTCACTAAGTGCATTG |
JQ806282 |
| Digalactosyldiacylglycerol synthase 1 (DGD1) |
I |
AGACCTGCATCTCTACCTCC |
GGTGCTGCCTAAATCTATATTC |
JQ806283 |
| Monogalactosyldiacylglycerol synthase (MGD2) |
I |
GTGTAAAGAATGGCAAGCATG |
CCCCTAAAAGAATCAGAAACC |
JQ806284 |
| Phosphatidylinositol synthase (PTS) |
I |
CTTTTCATCTTCTGTGTCCATG |
TAGTCAATAACCATCTCGTGC |
JQ806287 |
| Short-chain acyl-CoA oxidase (SCAOX) |
I |
AGGTTCTGCTTTTGCGCTAC |
GGTCCCCTAGCTGGTAATTC |
JQ806289 |
| Long-chain acyl-CoA oxidase (LCAOX) |
I |
TGCACCAAGAGTATGATAGGC |
TTACGTTTCTTTGTTCCAGCC |
JQ806290 |
| Acyl-CoA oxidase (AOX) |
I |
CCGTAATGCAAGACTGTGAAG |
TTGCCATTAACTTGGATACAGC |
JQ806291 |
| Long-chain acyl-CoA synthetase (LACS) |
I |
CAAGAGAGAGGCCATCAGG |
AAAATCCAAGAGAAACAGCAAG |
JQ806292 |
| Oleosin 3 (Oleosin 3) |
I |
AAGAGAAGTGGGTTTTGGTGG |
AGAAACAAAAAGATTTAAGCG |
JQ806304 |
| Diacylglycerol kinase (DGK) |
I |
GTGGCTCAGATTTGGGTTGC |
AAACTATTGAAGCTAAGCCTGG |
JQ806306 |
| D-erythro-sphingosine kinase/diacylglycerol kinase (DeDGK) |
I |
ATCAAATTCAGGAAAAGTAGCG |
AATCAAACTGCACAAAAGGAAC |
JQ806308 |
| Calmodulin-binding diacylglycerol kinase (cDGK) |
I |
AGAAGATAAGGAAGAGCGAAG |
GTTATAGCCTACAGCCAAAGC |
JQ806309 |
| Protein phosphatase 2C (PP2C) |
I |
TCATGGGCTTAAATGTGTGTAC |
AACTCATACTTGAAAAGCTAAGG |
JQ806311 |
| Tyrosine phosphatase (TP) |
I |
TGGCCGTTTGTTAAG ATTGATC |
ATTGACTTCATAATGTTGACCC |
JQ806312 |
| Phosphoglycolate phosphatase (PGP) |
I |
ATGCTGATGGGCTTTACTTTG |
AGAACTAGCAAACTCCTTCCC |
JQ806314 |
| Lysophosphatidyl acyltransferase 1(LPAT1) |
I |
CTGAAGGTTAGTGCAACAAATG |
GTAACATCGTCTGGAAAATTGC |
JQ806317 |
| Lysophosphatidyl acyltransferase 2 (LPAT2) |
I |
CTTTGGTTTCATGTGCTGCAC |
ATACATGAAAAGAAAAGGTGCC |
JQ806320 |
| Lysophosphatidyl acyltransferase 5 (LPAT5) |
I |
TTTGGCATCTGCAACCTATTTC |
GCCATAAACAGGTATGAGTCTC |
JQ806319 |
| Diacylglycerol acyltransferase (DGAT1) |
I |
GACCTAATGAATCGGAAAGGC |
CCGCATAGCCAAAATTGCTTG |
JQ806316 |
| Phospholipid/glycerol acyltransferase (PL/GA) |
I |
TAAAGTATTCTCGCCCTAGCCC |
ACATTTGCTTCTGTTTTCATGC |
JQ806322 |
| Triacylglycerol lipase (TAGL) |
I |
GTGAATACTGTTGTAAGCCTG |
GTCCAAAAACACCAATGAAATG |
JQ806324 |
| Phospholipase C (PLC) |
I |
CAGCTCAATGGTGATTATGTC |
AGCTTTTATGTAATTTGCGTCG |
JQ806325 |
| Phospholipase D (PLD) |
I |
ACTATGGGCAGAGCATGTTG |
TCACATCCAGGAATAGCCTC |
JQ806326 |
| Phospholipase D α (PLDα) |
I |
CGCCAAATCTGATTACCTCC |
CACTGATATGAACATCCTGGC |
JQ806327 |
| Phosphatidylinositol 4-kinase (P4K) |
I |
AAGACTTCTAGGGTTTGTGGG |
CTCCTCAGTCCTCACTTAGC |
JQ806329 |
| Putative phosphatase (PP) |
I |
TGGGAAGATGCCATGTCTATC |
CCAAACAAGAGATAAACTAACAG |
JQ806330 |
| Fatty acid desaturase (FAD) |
I |
ATGACCAATCCTGTTCCAAAG |
TGCTAATGTTTACAAATGAGGG |
JQ806294 |
| Fatty acid desaturase 5 (FAD5) |
I |
ACTTGGTATGTTGTGAGGTTTC |
ATGTAGAAAAGCTAATGCCCC |
JQ806295 |
| Fatty acid desaturase 6 (FAD6) |
I |
TTGCCCCTGAAGAATCTCAAC |
ATTCATATTACTGTCCTCCCC |
JQ806296 |
| Acyl-ACP desaturase (AAD) |
I |
AGTTTTTGATCGGACGGTGG |
AAAGAGAAGAAAGCAAGACTCG |
JQ806300 |
| δ-12-acyl-lipid desaturase (DALD) |
I |
GCTATAATATGTGGTTTGGCC |
GTTGTAGAGTTCCATAAACGG |
JQ806301 |
| δ-7-C-5 sterol desaturase (D7SD) |
I |
TCTTGCATAGGCATCCATTTC |
AGATCGAGTACATGGCTATGG |
JQ806302 |
| δ-9-stearoyl-acyl carrier protein desaturase (D9SD) |
I |
TGTTTGGAGAAGACATACCGG |
CAGGGCTGTGGTGACTTAC |
JQ806303 |
| δ-12 fatty acid conjugase (D12FAC) |
I |
AGCCAGAAGAGGGAGGTCC |
AACTCAAACACCACTTTCCCG |
JQ806298 |
| Fatty acid desaturase 2 (FAD2) |
I |
TGGACTACTTGTTAGGAATTTG |
GTTCATATTGTTTCTACCTGGG |
JQ806297 |
| Sterol desaturase (SD) |
II |
CTGATGAAGGCACACTGTTG |
GTAGAAACATAATCCACTGCC |
JQ806299 |
| 3-ketoacyl-CoA reductase isoform 2 (KCR2) |
II |
CGTCTCTCTCTCGGAATCC |
TCTTCTGTGAAAACGACCCTC |
JQ806265 |
| Enoyl-CoA hydratase (ECH) |
II |
AGATTGGAGGAATGGTCAAAG |
TATTGCTTGCTAGGATTGGAG |
JQ806267 |
| Oleosin (Oleosin) |
II |
TGGACAGTATATGCAACACAAG |
TATTCCACACTGAAATTAGCAC |
JQ806305 |
| Long-chain acyl-CoA synthetase 8 (LACS8) |
II |
TTTGTTTAATGTGCTTTCCTCC |
GTCCTGCAATTTAGGTGAAGC |
JQ806293 |
| Diacylglycerol kinase 1 (DGK1) |
II |
TGGCACTTAGGCTGACTTAG |
CAGCTAAAAGCACCAAGTTAAG |
JQ806307 |
| Lysophosphatidyl acyltransferase 4 (LPAT4) |
II |
ATTTTAGCATGTGCATTCCTTG |
TATAAACAAGTTCACAAAAAGGTC |
JQ806318 |
| Lipase (Lipase) |
II |
TGGGGTTCAATGCCAAAGAC |
TAGCCTGTCTACAGATTTTCC |
JQ806323 |
| Ketoacyl-CoA synthase (KCS) |
III |
CTTTGATTTGTACTTTCATGGG |
AACACACAAGCATTTGAAGCC |
JQ806268 |
| Choline kinase (CLK) |
III |
TATTTCTTCCTGCGATACAATG |
TTTGGATCTTAAATCTGGCTAC |
JQ806313 |
| Protein phosphatase 2A (PP2A) |
IV |
TGATGTTACCCGTAGAACTCC |
ATAAAAAAAAACAAAACCTGCCC |
JQ806310 |
| Ketoacyl ACP reductase (KAR) |
IV |
CGGAAGAGGTTGCAGGATTG |
AAAAACTGCCTCAACACAAGC |
JQ806278 |
| Phosphatidic acid phosphatase α (α-PAP) |
IV |
ATGGGGCTATATTTGGCTCAC |
CTTGGAAACCTGATAAACAAAAG |
JQ806280 |
| Cholinephosphate cytidylyltransferase (CPC) |
IV |
GACAAAGATGATGCTAAGGAG |
TATTCCTATCCTCACAACAAGC |
JQ806285 |
| Cyclopropane-fatty-acyl-phospholipid synthase (CFAS) |
IV |
GACTTGTCTTCCTGAGAGCC |
GCAGTTCTTTGAAAGCGATGG |
JQ806286 |
| Sulfoquinovosyldiacylglycerol synthase type 2 (SQD2) |
IV |
GCAGCCACTAGAAAAATCCG |
GCAGAGCAAATCCGTCACC |
JQ806288 |
| Phosphotyrosine protein phosphatase (PPP) |
IV |
ATGTGCTCCTTTTGTAAGAAAC |
AACTTTGAATGCCGCTGGTC |
JQ806315 |
| Glycerol-3-phosphate acyltransferase (G3PAT) |
IV |
GGCAGTAATGTCTTTGGTTTC |
TACATGAAAAGAAAGGGTGCC |
JQ806321 |
| Phosphatidylinositol 3-kinase (P3K) |
IV |
TGGAGTTTGCTCAAGGAGTC |
TTAAAAAAGAGCTGAAAGACACC |
JQ806328 |
| Malonyl CoA ACP transacylase (MCAT) |
V |
GTTATTGCTGGCATTGTCAAG |
GAAATCTCTAGTACATGACGC |
JQ806279 |
| Lipid phosphate phosphatase 3 (LPP3) |
V |
TCTGAGACGAGCGGAGGAC |
ATTCATCATCTCCTTCCAGTTC |
JQ806270 |
| Jatropha Actin 1 protein (Actin1) | Control | TAATGGTCCCTCTGGATGTG | AGAAAAGAAAAGAAAAAAGCAGC | JQ806331 |
1The genes can be grouped into five categories based on their expression patterns during endosperm development. Please refer to the text for details.