Table 3. Genetic polymorphism data for mitochondrial DNA sequences from the Pristimantis populations in this study.

Loci	Species	Population	N	h	S	θW	π	DT	DT 95% I.C.	Prob. DT≠0 Simul. Coal.
16S	P. brevifrons	Paraguas	7	2	1	0.00076	0.00053	−1.0062	−1.0062 to 1.341	0.490
		Peñas Blancas	12	4	3	0.00186	0.00094	−1.6292	−1.629 to 1.972	0.543
	P. jubatus	Observatorio-Santa Ana-Charguayaco	30	4	19	0.01047	0.0029	−2.5212*/−1.248	−1.712 to 1.816/−1.509 to 2.014	0.000
	P. palmeri	Paraguas	7	1	0	0	0	N.A.	N.A.	N.A.
		Chicoral	14	4	4	0.00235	0.00152	−1.1644	−1.797 to 1.886	0.1440
		Peñas Blancas	11	1	0	0	0	N.A.	N.A.	N.A.
COI	P. brevifrons	Paraguas	6	2	3	0.00201	0.00153	−1.2331	−1.233 to 1.909	0.268
		Peñas Blancas	10	2	1	0.00057	0.00032	−1.1117	−1.111 to 1.463	0.412
	P. jubatus	Observatorio-Santa Ana-Charguayaco	31	4	48	0.02036	0.00604	−2.6023*/0.2368	−1.719 to 1.792/−1.507 to 2.027	0.000
	P. palmeri	Paraguas	7	3	2	0.00132	0.0017	1.1684	−1.237 to 1.649	0.522
		Chicoral	15	7	10	0.00498	0.0045	−0.3644	−1.802 to 1.827	0.554
		Peñas Blancas	13	5	94	0.04901	0.04527	−0.3921	−1.760 to 1.700	0.543

N  =  number of sequences, h  =  number of haplotypes, S  =  segregating sites, θ_W  =  population mutation rate per site, π  =  nucleotide diversity per site, D_T  =  Tajima’s D statistic [73]. An asterisk (*) in the D_T column indicates a significant deviation from the expected value under neutrality (D_T = 0) as calculated from coalescence simulations. D_T values in bold obtained for P. jubatus when the divergent sample UVC15842 was excluded. N.A.  =  Not Applicable. Indels and sites with missing data were not taken into account in pairwise comparisons.