Figure 6. Parameter A and phase diagram analyses of intrinsic fluorescence data.

(A) Parameter A was produced by dividing the fluorescence intensity at 320 nm (I₃₂₀) by the intensity at 365 nm (I₃₆₅). (B) In the phase diagram analysis, the fluorescence data were normalized by the I₃₂₀ and I₃₆₅ of samples fully denatured in 4 M GdnHCl. The data used was from Fig. 3. Only WT CK (filled square), H26Y (open square), P36T (filled circle) and T59I (open circle) are shown in (B); other mutants are the same as WT CK. Inset are enlargements of Parameter A at low GdnHCl concentrations.