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. 2012 Sep 25;7(9):e45955. doi: 10.1371/journal.pone.0045955

Figure 3. Specific extraction at the P224 locus by HSE and evaluation by AmpFℓSTR® Yfiler PCR.

Figure 3

(A) Map of the 11 kb distant DYS390 and P224 loci on the human Y chromosome, which were selected for HSE analysis. Arrows indicate the positions of the probes used (RG, FC, FT, or RA), which were orientated in either the forward (F) or reverse (R) direction. Given chromosome positions are from a reference sequence (Hg18). (B) Electropherograms of AmpFℓSTR® Yfiler analysis of haplo-separated samples obtained from a DNA mixture (Contributor 1 and Contributor 2) using the probes P224FC (23 and 19 nucleotides long) and P224FT (23 and 20 nucleotides long), or without probes. Green bars with allele 23 correspond to Contributor 1, and brown bars with allele 24 correspond to Contributor 2. Percentages indicate the enrichment of one Contributor.