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. 2012 Sep 25;7(9):e45971. doi: 10.1371/journal.pone.0045971

Table 2. List of the combination of fluorochrome-conjugated anti-human mAbs used for FCM analysis.

Cell subpopulation Combination of fluorochrome-conjugated mAbs
FITC RPE RPE-Cy5 APC
CD4+ MemoryT cells anti-CD45ROa anti-CD4a anti-CD3a
CD8+ MemoryT Cells anti-CD45ROa anti-CD8a anti-CD3a
Gamma deltaT cells anti-gamma9b anti-CD3a
B cells anti-CD19a anti-CD3a
Regulatory T cells anti-Foxp3c anti-CD25b anti-CD3a anti-CD4a
NK and NK T cells anti-CD56a anti-CD3a
a

Caltag, Burlingame, USA;

b

Immunotech, Marseille, France.

c

This mAb was conjugated with Alexa fluor® 488 (BioLegend, San Diego, USA).