Table 2. List of the combination of fluorochrome-conjugated anti-human mAbs used for FCM analysis.
Cell subpopulation | Combination of fluorochrome-conjugated mAbs | |||
FITC | RPE | RPE-Cy5 | APC | |
CD4+ MemoryT cells | anti-CD45ROa | anti-CD4a | anti-CD3a | |
CD8+ MemoryT Cells | anti-CD45ROa | anti-CD8a | anti-CD3a | |
Gamma deltaT cells | anti-gamma9b | anti-CD3a | ||
B cells | anti-CD19a | anti-CD3a | ||
Regulatory T cells | anti-Foxp3c | anti-CD25b | anti-CD3a | anti-CD4a |
NK and NK T cells | anti-CD56a | anti-CD3a |
Caltag, Burlingame, USA;
Immunotech, Marseille, France.
This mAb was conjugated with Alexa fluor® 488 (BioLegend, San Diego, USA).