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. 2012 Sep 25;7(9):e46251. doi: 10.1371/journal.pone.0046251

Figure 5. FET-protein reduction is not affecting ROS.

Figure 5

(A) HEK293 cells were transfected with siRNAs targeted against the FUS, EWS, or TAF15 mRNAs, or with an unspecific siRNA as control. Cells were also transfected with a combination of all three FET siRNAs (300 nM total concentration) or unspecific siRNA at concentration of 300 nM (lower panel). The level of ROS in the cells was measured and evaluated by microscopy. Untransfected cells treated with TBHP is a positive control of oxidative stress (upper panel left), and untransfected cells without TBHP treatment is a negative control of oxidative stress (upper panel right). (B) HEK293 cells were transfected with a combination of all three FET siRNAs (300 nM total) or with an unspecific siRNA as control. Cells were subsequently treated with TBHP to induce oxidative stress (left columns), and the level of ROS was measured and evaluated by microscopy and compared to unstressed cells (right columns). Untransfected cells treated with TBHP is a positive control of oxidative stress (upper panel left), and untransfected cells without TBHP treatment is a negative control of oxidative stress (upper panel right).