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. 2012 Sep 25;7(9):e45851. doi: 10.1371/journal.pone.0045851

Figure 2. Effect of serum dilution on the detection of anti-WNV IgM and IgG antibodies in mice.

Figure 2

NHI and HI sera at indicated time-points after infection were diluted serially from 1∶20 to 1∶160 in PBS-1% BSA and were tested by WNV E-MIA for the presence of anti-WNV (A and B) IgM and (C and D) IgG antibodies. Data are expressed as MFI ± SD and is representative of two independent experiments conducted in duplicate. Dotted line indicates the cutoff value. HI serum is depicted by red line and NHI serum by blue line.