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. 2012 Sep 25;7(9):e46253. doi: 10.1371/journal.pone.0046253

Figure 7. SU5416 reduces the expression of nNOS protein elevated by MPP+ in CGNs.

Figure 7

(A) CGNs were pre-treated with 20 µM SU5416 or DMSO (vehicle control) for 2 hours, and then treated with 35 µM MPP+ for various durations as indicated. The total proteins were extracted for Western blot analysis with specific iNOS, nNOS and β-actin antibodies. (B) Statistical analysis of nNOS expression in each treatment group. Data are expressed as the ratio to OD values of the corresponding controls. Data, expressed as percentage of control, were the mean ± SEM of five separate experiments; *p<0.05 versus MPP+ group at the same time (Turkey’s test).